richard pagano (Addgene inc)

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Addgene inc richard pagano
Richard Pagano, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 57 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/richard+pagano/DsRed-rab11+WT+(Plasmid+%2312679)/pm40586707-132-6-8
Average 93 stars, based on 57 article reviews

richard pagano - by Bioz Stars, 2026-11

93/100 stars

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Plasmid Preparation:

Article Title: Ubiquitination of the protocadherin-γA3 variable cytoplasmic domain modulates cell-cell interaction.
Article Snippet: Point mutations within the γA3 stub and full length Pcdh-γA3 were generated using the Agilent QuikChange Site Directed Mutagenesis Kit (Agilent Technologies). .. GFP-Rab5B was a gift from Gia Voeltz (Addgene plasmid # 61802; http://n2t.net/ addgene:61802; RRID:Addgene_61802), GFP-Rab7A was a gift from Gia Voeltz (Addgene plasmid # 61803; http://n2t.net/ addgene:61803; RRID:Addgene_61803), GFP-rab11 WT was a gift from Richard Pagano (Addgene plasmid # 12674; http://n2t. net/addgene:12674; RRID:Addgene_12674), mRFP-Ub was a gift from Nico Dantuma (Addgene plasmid # 11935; http://n2t.net/ addgene:11935; RRID:Addgene_11935). .. 24 h post-transfection, cells were washed with ice cold PBS and lysed with 1.2 mL ice cold RIPA buffer 1% Triton, 0.5% Sodium Deoxycholate, 150 mM NaCl, 0.1% SDS, 50 mM Tris pH7.7, 2 mM EDTA, 10 mMN-Ethylmaleimide, and protease inhibitor (Complete Mini, Roche) on ice for 20 min with agitation.

Article Title: Regulation of CTLA-4 recycling by LRBA and Rab11.
Article Snippet: .. The following Rab- GFP constructs were acquired from Addgene and generated by Marci Scidmore (Rzomp et al; Cortes et al) (Rab4 WT, DN, CA; Rab5 WT; Rab11 CA), Sergio Grinstein (Bohdanowicz et al) (Rab5 DN, CA) and Richard Pagano (Choudhury et al) (Rab9 WT, DN; Rab11 WT, DN): Rab4WT (Addgene plasmid #49434), Rab4 DN (#49476), Rab4 CA (#49475), Rab5 WT (#49888), Rab5 DN (#35141), Rab5 CA (#35140), Rab9 WT (#12663), Rab9 DN (#12664), Rab11 WT (# 12674), Rab11 DN (#12678) and Rab11 CA (#49553). ..

Article Title: Transmembrane protein 184B (TMEM184B) modulates endolysosomal acidification via the vesicular proton pump.
Article Snippet: FLAG-GFP was made in house. mCherry-Rab7a-7 was a gift from Michael Davidson (Addgene plasmid # 55127; http://n2t.net/addgene:55127; RRID: Addgene_55127). mCherry-Rab5a-7 was a gift from Michael Davidson (Addgene plasmid # 55126; http://n2t.net/addgene:55126; RRID: Addgene_55126). .. DsRed-rab11 WT was a gift from Richard Pagano (Addgene plasmid # 12679; http://n2t.net/addgene:12679; RRID: Addgene_12679). pLJM1-FIRE-pHLy was a gift from Aimee Kao (Addgene plasmid # 170775; http://n2t.net/addgene:170775; RRID: Addgene_170775). .. HEK293T cells were cultured in high glucose Dulbecco’s Modified Eagle Medium (DMEM; Gibco) containing 10% fetal bovine serum, 110mg/mL sodium pyruvate, and 10,000 U/mL Penicillin/Streptomycin.

Article Title: Regulation of CTLA‐4 recycling by LRBA and Rab11
Article Snippet: .. The following Rab‐GFP constructs were acquired from Addgene and generated by Marci Scidmore (Rzomp et al; Cortes et al) (Rab4 WT, DN, CA; Rab5 WT; Rab11 CA), Sergio Grinstein (Bohdanowicz et al) (Rab5 DN, CA) and Richard Pagano (Choudhury et al) (Rab9 WT, DN; Rab11 WT, DN): Rab4WT (Addgene plasmid #49434), Rab4 DN (#49476), Rab4 CA (#49475), Rab5 WT (#49888), Rab5 DN (#35141), Rab5 CA (#35140), Rab9 WT (#12663), Rab9 DN (#12664), Rab11 WT (# 12674), Rab11 DN (#12678) and Rab11 CA (#49553). ..

Clone Assay:

Article Title: Mechanisms of aquaporin-4 vesicular trafficking in mammalian cells.
Article Snippet: Plasmids: mCherry- Rab5a- 7 (RRID:Addgene_55126) and mCherryRab11a- 7 plasmids (RRID:Addgene_55124) were a gift from Michael Davidson. .. DsRed- rab11DN(S25N) was a gift from Richard Pagano (RRID:Addgene_12680); mCherry- Rab5DN(S34N) was a gift from Sergio Grinstein (RRID:Addgene_35139); Human AQP4 was cloned into mammalian expression vector pDEST47 (Invitrogen, Cat. nr. 12281010). ..

Expressing:

Construct:

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other:

Article Title: Transmembrane protein 184B (TMEM184B) modulates endolysosomal acidification via the vesicular proton pump.
Article Snippet: FLAG-hTMEM184B-GFP was generated by Twist Biosciences.

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